The efficacy of hibiscus rosa-sinensis leaf extracts against candida SPP, causing candidiasis
Over the years, World Health Organization has recommended traditional medicines as safe remedies for ailments of both microbial and non-microbial origins. The use of herbals has increased worldwide in recent years, as they are believed to be safer than modern medicines with lesser or no side effects. The present study has been designed to determine the role of Hibiscus rosa-sinensis leaves extract in-vitro antifungal activities against Candida albicans, Candida tropicalis, and Candida glabrata species. The leaves of H. rosa-sinensis were soaked in methanol and ethanol for solvent extraction. The extraction yield was diluted in DMSO to obtain 6 different concentrations, and was then impregnated on blank discs. The disc diffusion method was used to test against 3 different candida species for sensitivity test. Basic phytochemical constituent’s detection on phenol, carbohydrate, tannins and saponins were done on H. rosa-sinensis. The extract that shows largest zone of inhibition of candida species is the leaf extract with ethanol. 1000mg/ml of the leaf extract shows the highest inhibition zone against C. albicans, C. tropicalis and C. glabrata which are 9mm, 8mm and 9mm respectively. Carbohydates test showed both extractions highly positive and ethanol extract exhibited the highest saponin compared with methanol extract. It was found that ethanol extract of H. rosa- sinensis showed more potent anti candidiasis compared with methanol extract.
Chen LM, Xu YH, Zhou CL, Zhao J, Li CY, Wang R. (2010) Over expresion of CDR1 and CDR2 genes plays an important role in fluconazol resistance in Candida albicans with G487T and T916C mutations. J Int Med Res 2010;38:536e45.
Collin B, Clancy CJ, Hong Nguyen M. (1999) Antifungal resistance in non-albicans candida species. Drug Resist Updates 1999;2:9e14.
Chandha S.,Dave R. (2009) In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. African Journal of Microbiology Research. 2009; 3:981–96.
Chang C, Wen H, Yang M. (2002) Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food and Drug Analysis. 2002; 10:178–82.
David, B and L.A, Leonard. (1998) Healing plants of the Hawaiian kingdom- Hibiscus rosa sinensis (Aloalo), Roast Duck Production.
Dixon, T. C., Steinbach, W. J., Benjamin, D. K., Jr, Williams, L. W. & Myers, L. A. (2004). Disseminated Candida tropicalis in a patient with chronic mucocutaneous candidiasis. South Med J 97, 788– 790.
Jayaraman J. (2011) Estimation of Chlorophyll and Carotenoids. In Laboratory Manual in Biochemistry. New Age International 2011; pp: 141–2.
Komshian S V, Uwaydah A K, Sobel J D. (1999) Fungemia caused by Candida species and Torulopsis glabrata in the hospitalized patient: frequency, characteristics, and evaluation of factors influencing outcome. Rev Infect Dis. 1999;11:379–390.
Kumar S, Narain S. (2010) Herbal remedies of wetlands macrophytes in India. Int J Pharm BioSci 2010;1(2): 1-12
Lalit K., Gunosindhu C, Vjay S and Avijit M. (2012) Hibiscus rosa-sinensis : A review on divine herb. J.Adv. Pharm. Health Res 2012; 2(4): 9-18.
McDonald S, Prenzler P, Robards K. (2001) Phenolic content and antioxidant activity of olive extracts. Food Chemistry. 2001; 73:73–84.
Nicholls S, MacCallum DM, Kaffarnik FA, Selway L, Peck SC, Brown AJ (2011). Activation of the heat shock transcription factor Hsf1 is essential for the full virulence of the fungal pathogen Candida albicans.. Fungal Genet Biol 2011; 48:297 - 305
Obi, F.O., Usenu, I.A., Osayande, J.O. (1998) Prevention of carbon tetrachloride-induced hepatotoxicity in the rat by H. rosa sinensis anthocyanin extract administered in ethanol.Toxicology 131, 93– 98.
Pamplona-Roger, M.D. (1999) Encyclopedia of medicinal plants. Madrid (Spain): Editorial Safeliz, S.L. Vol. 1, 781p.
Turkmen N., Sari F., Velioglu Y.S. (2006) Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin–Ciocalteu methods. Food Chem, 99 (2006), pp. 835-841
Udita-Tiwari, Yadav, P., and Nigam, D. (2015). Study on Phytochemical Screening and antibacterial Potential of Methanolic Flower and Leaf Extracts of Hibiscus rosa- sinensis. Int. J. Innovative and App. Res., 3(6): 9-14