Prevalence and associated risk factors of Entamoeba histolytica, E. dispar and E. moshkovskii infection among Orang Asli communities in Slim River, Perak

  • Syazwan Saidin Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Tanjong Malim, Perak, Malaysia
  • Adibah Abu Bakar Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Tanjong Malim, Perak, Malaysia
  • Badrul Munir Mohd Zain School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia
Keywords: Amoebiasis, Entamoeba, Orang Asli, epidemiology, E. histolytica; E. dispar


Entamoeba infection is still widespread in Malaysia’s rural area particularly among Orang Asli communities which usually associated with poverty and lack of sanitation. Due to scarce information on these Entamoeba infections in Slim River, Perak we conducted this study to investigate the prevalence and associated risk factors towards this infection. A total of 55 stool samples from voluntary participants with and without symptoms of amoebiasis were collected and examined using PCR technique. PCR analysis showed 16.4% stool samples were detected positive for Entamoeba spp., discriminated as 7.3% that were positive for E. histolytica and 9.1% for E. dispar. No E. moshkovskii was detected at all. Factors such as indiscriminate defecation, improper sewage disposal and not washing hand after playing with soil or gardening showed significant association with E. histolytica infection; while gastrointestinal symptom such as vomiting was associated with E. dispar infection. In conclusion, the study reveals there is still an occurrence of Entamoeba spp. among Orang Asli communities in Slim River, Perak. This implies that good personal and hygiene practices should be enhanced through awareness strategy to control Entamoeba infections in Orang Asli communities in Malaysia.


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How to Cite
Saidin, S., Abu Bakar, A., & Mohd Zain, B. M. (2020). Prevalence and associated risk factors of Entamoeba histolytica, E. dispar and E. moshkovskii infection among Orang Asli communities in Slim River, Perak. Journal of Science and Mathematics Letters, 8(2), 22-35. Retrieved from