TY - JOUR AU - Jibril, Saidu AU - Sirat, Hasnah Mohd AU - Idris, Maikudi Musa AU - Mohammad, Aminu AU - Muhammad, Kamal Ja’afar PY - 2020/03/10 Y2 - 2024/03/19 TI - Ultrasonic Assisted Extraction and Gas Chromatography-Tandem Mass Spectroscopy (GC-MS/MS) Analysis of Extracts from Four Cassia Species JF - Journal of Science and Mathematics Letters JA - JSML VL - 8 IS - 2 SE - Articles DO - 10.37134/jsml.vol8.2.1.2020 UR - https://ejournal.upsi.edu.my/index.php/JSML/article/view/3122 SP - 1-7 AB - <p style="text-align: justify;">Ultrasonic assisted extraction (UAE) process increases the rate of extraction, the yield of extracted components and reduces extraction time. The high sensitivity of GC-MS/MS provides reliable identification of phytoconstituents present in a plant extract. This research is to develop an easy and effective method for the extraction and analysis of phytochemicals in <em>C</em><em>. singueana</em>, <em>C. sieberiana</em>, <em>C. tora </em>and <em>C. occidentalis </em>using UAE and GC-MS/MS techniques. The crude extracts obtained from the four <em>Cassia </em>species under UAE conditions of 50 mins extraction time, 50 <sup>o</sup>C extraction temperature and 20 mL/g solvent to sample ratio were analyzed using GC-MS/MS. Thirty-four compounds were identified by comparison of their mass spectra with those in the National Institute of Standards and Technology library, requiring at least 80% similarity. These compounds comprise fatty acids, fatty alcohol, carboxylic acid, long-chain alkanes, diterpenes, triterpenes, sterols and anthraquinones. <em>n</em>-Hexadecanoic acid and phytol were identified in all the <em>Cassia</em> species while anthraquinone was detected only from the methanol extracts of <em>C. tora</em> and <em>C. occidentalis.</em> The bioactivities reported in the literature for these compounds corroborate with the phytoconstituents identified in these plants and support the ethnomedicinal uses of these <em>Cassia</em> species.</p> ER -