Synergistic Effects of Radiotherapy and Capsaicin on Colorectal Cancer (CaCo-2) and Normal Fibroblast (HDFn) Cell Lines In Vitro
DOI:
https://doi.org/10.37134/Keywords:
Colorectal cancer, CaCo-2, HDFn, Capsaicin extract, radiotherapyAbstract
Colorectal cancer is the second most frequent kind of cancer worldwide. The treatment may comprise surgery, chemotherapy, and radiation therapy. More than half of cancer treatments are derived from natural sources, such as capsaicin. The biggest challenge is to get the disease under control as much as possible while causing as little harm to the healthy tissues around it. Cells were treated with an extract, Capsaicin of (chili peppers) and irradiated at 1 Gy, 3 Gy and 5 Gy. The MTT test was used to determine viability, and absorbance was measured at 570 nm using a Counter reader (Mutiskan EX, Thermo Lab systems). Capsaicin extract has shown a potential radiosensitizing effect in vitro for colorectal cancer. The inhibition effect in the cell lines was not selective and is significantly different from conventional radiotherapy. Capsaicin extract from chili peppers concerted with radiation proved more effective in colorectal cancer cells than radiotherapy alone.
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[1] Bray, F., Laversanne, M., Weiderpass, E., & Soerjomataram, I. (2021). The ever‐increasing importance of cancer as a leading cause of premature death worldwide. Cancer, 127(16), 3029– 3030.
[2.] Torre, L. A., Bray, F., Siegel, R. L., Ferlay, J., Lortet‐Tieulent, J., & Jemal, A. (2015). Global cancer statistics, 2012. CA: A Cancer Journal for Clinicians, 65(2), 87–108.
[3] Santandreu, F. M., Valle, A., Oliver, J., & Roca, P. (2011). Resveratrol potentiates the cytotoxic oxidative stress induced by chemotherapy in human colon cancer cells. Cellular Physiology and Biochemistry, 28(2), 219–228.
[4] van der Stok, E. P., Spaander, M. C. W., Grünhagen, D. J., Verhoef, C., & Kuipers, E. J. (2017). Surveillance after curative treatment for colorectal cancer. Nature Reviews Clinical Oncology, 14(5), 297–315.
[5] Park, S.-Y., Kim, J.-Y., Jang, G.-B., Choi, J.-H., Kim, J.-H., Lee, C.-J., Lee, S., Baek, J.-H., Park, K.-K., & Kim, J.-M. (2021). Aberrant activation of the CD45-Wnt signaling axis promotes stemness and therapy resistance in colorectal cancer cells. Theranostics, 11(18), 8755.
[6] Fernández, J., Redondo-Blanco, S., Gutiérrez-del-Río, I., Miguélez, E. M., Villar, C. J., & Lombo, F. (2016). Colon microbiota fermentation of dietary prebiotics towards short-chain fatty acids and their roles as anti-inflammatory and antitumour agents: A review. Journal of Functional Foods, 25, 511–522.
[7] Pan, M., Lai, C., Wu, J., & Ho, C. (2011). Molecular mechanisms for chemoprevention of colorectal cancer by natural dietary compounds. Molecular Nutrition & Food Research, 55(1), 32–45.
[8] Housman, G., Byler, S., Heerboth, S., Lapinska, K., Longacre, M., Snyder, N., & Sarkar, S. (2014). Drug resistance in cancer: an overview. Cancers, 6(3), 1769–1792.
[9] Colussi, D., Brandi, G., Bazzoli, F., & Ricciardiello, L. (2013). Molecular pathways involved in colorectal cancer: implications for disease behavior and prevention. International Journal of Molecular Sciences, 14(8), 16365–16385.
[10] Singh, C. K., George, J., & Ahmad, N. (2013). Resveratrol‐based combinatorial strategies for cancer management. Annals of the New York Academy of Sciences, 1290(1), 113–121.
[11] Amiri, F., Zarnani, A.-H., Zand, H., Koohdani, F., Jeddi-Tehrani, M., & Vafa, M. (2013). Synergistic anti-proliferative effect of resveratrol and etoposide on human hepatocellular and colon cancer cell lines. European Journal of Pharmacology, 718(1–3), 34–40.
[12] Fattorusso, E., & Taglialatela-Scafati, O. (2007). Modern alkaloids: structure, isolation, synthesis, and biology. John Wiley & Sons.
[13] Perucka, I., & Oleszek, W. (2000). Extraction and determination of capsaicinoids in fruit of hot pepper Capsicum annuum L. by spectrophotometry and high-performance liquid chromatography. Food Chemistry, 71(2), 287–291.
[14] Koleva-Gudeva, L., Mitrev, S., Maksimova, V., & Spasov, D. (2013a). Content of capsaicin extracted from hot pepper (Capsicum annuum ssp. microcarpum L.) and its use as an ecopesticide. Hemijska Industrija, 67(4), 671–675.
[15] Koleva-Gudeva, L., Mitrev, S., Maksimova, V., & Spasov, D. (2013b). Content of capsaicin extracted from hot pepper (Capsicum annuum ssp. microcarpum L.) and its use as an ecopesticide. Hemijska Industrija, 67(4), 671–675.
16) Chen, G., Han, Y., Zhang, H., Tu, W., & Zhang, S. (2021a). Radiotherapy-induced digestive injury: diagnosis, treatment and mechanisms. Frontiers in Oncology, 11, 757973.
[17] Lomax, M. E., Folkes, L. K., & O’neill, P. (2013). Biological consequences of radiation- induced DNA damage: relevance to radiotherapy. Clinical Oncology, 25(10), 578–585.
[18] Chen, G., Han, Y., Zhang, H., Tu, W., & Zhang, S. (2021b). Radiotherapy-induced digestive injury: diagnosis, treatment and mechanisms. Frontiers in Oncology, 11, 757973.
[19] Qu, W., Zhang, L., & Ao, J. (2020). Radiotherapy induces intestinal barrier dysfunction by inhibiting autophagy. Acs Omega, 5(22), 12955–12963.
[20] Chung, D. M., Kim, J. H., & Kim, J. K. (2015). Evaluation of MTT and Trypan Blue assays for radiation-induced cell viability test in HepG2 cells. International Journal of Radiation Research, 13(4), 331.
[21] Shukla, P. K., Gangwar, R., Manda, B., Meena, A. S., Yadav, N., Szabo, E., Balogh, A., Lee, S. C., Tigyi, G., & Rao, R. (2016). Rapid disruption of intestinal epithelial tight junction and barrier dysfunction by ionizing radiation in mouse colon in vivo: protection by N-acetyl-l- cysteine. American Journal of Physiology-Gastrointestinal and Liver Physiology, 310(9), G705–G715.
[22] Suetens, A., Moreels, M., Quintens, R., Soors, E., Buset, J., Chiriotti, S., Tabury, K., Gregoire, V., & Baatout, S. (2015). Dose-and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation. Journal of Radiation Research, 56(1), 11–21.
[23] Lee, S.-H., Krisanapun, C., & Baek, S. J. (2010). NSAID-activated gene-1 as a molecular target for capsaicin-induced apoptosis through a novel molecular mechanism involving GSK3β, C/EBPβ and ATF3. Carcinogenesis, 31(4), 719–728.
[24] Kim, M. Y., Trudel, L. J., & Wogan, G. N. (2009). Apoptosis induced by capsaicin and resveratrol in colon carcinoma cells requires nitric oxide production and caspase activation. Anticancer Research, 29(10), 3733–3740.
[25] Yang, K., Pyo, J., Kim, G.-Y., Yu, R., Han, I., Ju, S., Kim, W., & Kim, B.-S. (2009). Capsaicin induces apoptosis by generating reactive oxygen species and disrupting mitochondrial transmembrane potential in human colon cancer cell lines. Cellular and Molecular Biology Letters, 14(3), 497–510.
[26] Zhang, S., Wang, D., Huang, J., Hu, Y., & Xu, Y. (2020). Application of capsaicin as a potential new therapeutic drug in human cancers. Journal of Clinical Pharmacy and Therapeutics, 45(1), 16–
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