Preparation and Characterisation of Carvacrol Encapsulated in Gellan Gum Hydrogel
DOI:
https://doi.org/10.37134/ejsmt.vol4.1.2.2017Keywords:
carvacrol, encapsulation, gellan gum hydrogel, thin film, natural compoundAbstract
Studies on plant materials as natural compound such as carvacrol (Carv) have gained much attention. Carv exhibits numerous potential as antimicrobial agent, food additives, antioxidant and etc. However, this free standing bioactive compound is unstable in the harsh environment conditions. Hence, the encapsulation technology provides protection to enhance the effectiveness in release manner. In this study, the preparation of Carv encapsulated in gellan gum hydrogel forming thin film (GG-Carv TF) was achieved by using 1.0 g of gellan gum at different concentrations of Carv (0.01-0.04 M). The FTIR spectra of GG-Carv TF revealed the combination of both functional groups from GG and Carv. The Carbon, Hydrogen and Nitrogen, CHN analysis further confirmed the encapsulation with the changes in the element percentage. Both swelling and degradation percentage increased with time and showed decreasing patterns in the range of 680.79-666.78 % and 26.83-19.15 % which can be observed as the concentration of Carv increased, respectively.
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References
Chami, F., Chami, N., Bennis, S., Bouchikhi, T., and Remmal, A. (2005). Oregano and clove essential oils induce surface alteration of Saccharomyces cerevisiae. Phytother. Res. 19:405- 408.
Arfa, B.A., Combes, S., Preziosi-Belloy, L., Gontard, N., and Chalier, P. (2006). Antimicrobial activity of carvacrol related to its chemical structure. Lett.in Appl. Microbiol. 43:149-154.
Oussalah, M., Caillet, S., Saucier, L., and Lacroix, M. (2007). Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food Control. 18:414-420.
Ravishankar, S., Zhu, L., Reyna-Granados, J., Law, B., Joens, L., and Friedman, M. (2010). Carvacrol and cinnamaldehyde inactivate antibiotic-resistant Salmonella enterica in buffer and on celery and oysters. J. Food
Prot. 73:234-240.
Perez-Conessa, D., Cao, J., Chen, L., Mclandsborough, L., and Weiss, J. (2011). Inactivation of Listeria
monocytogenes and Escherichia coli O157:H7 biofilms by micelle encapsulated eugenol and carvacrol. J. Food
Prot. 74:55-62.
Han J. H.,Krochta, J. M., Kurth, M. J., and Hsieh, Y. L. (2000). Lactitol-based poly (ether polyol) hydrogels for
controlled release chemical and drug delivery systems. Journal of Agricultural and Food Chemistry. 48(11),
5278-5282.
Kang D., Zhang, H. B., Nitta, Y., Fang, Y. P., & Nishinari, K. (2015). Gellan. Polysaccharides: Bioactivity and
Biotechnology. 1627-1682.
Chalier P., A.B. Arfa, L.P. Belloy and N. Gontard. (2007). Carvacrol losses from soy protein coated papers as a
function of drying conditions. Journal of Applied Polymer Science. 106: 611-620.
Pourjavadi A., Kurdtabar M. (2007). Collagen-based highly porous hydrogel without any porogen: Synthesis
and characteristics. European Polymer Journal. 43: 877-889.
Veldhuizen, E. J. A., Tjeerdsma-Van Bokhoven, J.L.M., Zweijtzer, C., Burt, S.A., and Haagsman, H.P. (2006).
Structural requirements for the antimicrobial activity of carvacrol. J. Agric. Food Chem. 54:1874-1879.
Oliveira J. T., Santos T. C., Martins L., Picciochi R, Marques A. P., Castro A. G. (2010). Gellan gum injectable
hydrogels for cartilage tissue engineering applications: in vitro studies and preliminary in vivo evaluation. Tissue
Eng Part A. 16 (1):343-353.
Daniela F. Coutinho, Shilpa V. S., HyeonghoS., João T. O., Manuela E. G., Nuno M. N., Ali K., and Rui L. R. (2010). Modified Gellan Gum hydrogels with tunable physical and mechanical properties. Journal Biomaterials.
31: 7494-7502.
