Detection of Porcine Based Materials in Processed Food Using Polymerase Chain Reaction Method
DOI:
https://doi.org/10.37134/jsml.vol6.6.2018Keywords:
Halal authentication, processed foods, Polymerase Chain ReactionAbstract
The detection of halal adulteration in processed food, particularly the presence of porcine based material, be it human or animal food is very important to safeguard the interest of Muslim consumers. Many techniques have been developed to determine such adulteration which can ascertain the halal status of food products. In this paper, the presence of porcine based materials in few processed foods namely meat products, candy products, and animal feeds obtained from Kota Samarahan, Sarawak, Malaysia were screened by using Polymerase Chain Reaction (PCR) method. Findings revealed that all processed meat samples showed a positive result of the presence of porcine DNA. A similar result was also obtained for a few samples of animal feeds and candy products. As such, further study is required to verify the results.
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References
Ambali, A.R., & Bakar, A.N. (2013). Halal food and products in Malaysia: people's awareness and policy implications. Intellectual Discourse, 21(1), 7-32.
Buang, A.H., & Mahmod, Z. (2012). Isu dan cabaran badan pensijilan halal di Malaysia". Jurnal Syariah, 20(3), 271-288.
Che Man, Y.B., Aida, A.A., Raha, A.R., & Son, R. (2007). Identification of pork derivatives in food products by species-specific polymerase chain reaction (PCR) for halal verification. Food Control, 18(7), 885-889.
Che Man, Y.B., Syahariza, Z.A., Mirghani, M.E.S., Jinap, S., & Bakar, J. (2005). Analysis of potential lard adulteration in chocolate and chocolate products using fourier transform infrared spectroscopy. Food Chemistry, 90(4), 15-819.
Che Man, Y.B., Syahariza Z. A., & Rohman, A. (2011). Discrimination analysis of selected edible fats and oils and those in biscuit formulation using FTIR spectroscopy. Food Analytical Method, 4(3), 404-409.
Eliasi, J.R. (2002). Kosher and halal: religious observances affecting dietary intakes. Journal of American Dietetic Association, 102(7), 911-913.
Farag, R.S., Abo-raya, S.H., Ahmed, F.A., Hewedi, F.M., & Khalifa, H.H. (1983). Fractional crystallization and gas chromatographic analysis of fatty acids as a means of detecting butterfat adulteration. Journal of the American Oil Chemists Society, 60(9), 1665-1667.
Marikkar, J.M.N., Ghazali, H.M., Che Man, Y.B., Peiris, T.S.G., & Lai, O.M. (2005). Distinguishing lard from other animal fats in admixtures of some vegetable oils using liquid chromatographic data coupled with multivariate data analysis. Food Chemistry, 91(1), 5-14.