Recycling HPLC for the Isolation of Styryl Lactone from Goniothalamus lanceolatus Miq
DOI:
https://doi.org/10.37134/jsml.vol11.2.12.2023Keywords:
Recycling HPLC, Goniothalamus, Goniothalamus lanceolatusAbstract
A unique plant from the rainforest of Sarawak, Malaysia, Goniothalamus lanceolatus Miq., was chosen for a phytochemical study based on its ethnomedicinal characteristics amongst the locals. Isolation and purification of a minor compound exhibiting similar retention characteristics was made possible by using preparative Recycling HPLC. Due to a recycle valve in the HPLC system, this method enables the unresolved peaks to recirculate into the column repeatedly until the peaks were successfully separated as pure entity. During the recycling process, solvent consumption is not needed. Recycling HPLC expediates the isolation and purification of complex mixtures in such a sustainable way. One styryl lactone was successfully isolated and identified as 6S,7S,8S-(-)-goniodiol.
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References
Alkofahi A, Ma WW, McKenzie AT, Byrn SR, McLaughlin JL. (1989). Goniotriol from Goniothalamus giganteus. Journal of Natural Products, 52, 1371-1373.
Aslam MS, Ahmad MS, Mamat AS, Ahmad MZ, Salam F. (2016). Goniothalamus: phytochemical and eEthnobotanical review. Recent Advances in Biology and Medicine, 2, 34-47.
Bihud NV, Rasol NE, Imran S, Awang K, Ahmad FB, Mai CW, Leong CO, Cordell GA, Ismail NH. (2019). Goniolanceolatins A-H, cytotoxic bisstyryllactones from Goniothalamus lanceolatus. Journal of Natural Products, 82(9), 2430-2442.
Bihud NV. (2020). Styryllactones and bis-styryllactones from the stembark of Goniothalamus lanceolatus Miq. and their cytotoxic activity. PhD Thesis, Universiti Teknologi MARA (UiTM).
Lan YH, Chang FR, Yu JH, Yang YL, Chang YL, Lee SJ, Wu YC. (2003). Cytotoxic styrylpyrones from Goniothalamus amuyon. Journal of Natural Products, 66, 487-490.
Liou JR, Wu TY, Thang TD, Hwang TL, Wu CC, Cheng YB, Chiang MY, Lan YH, El-Shazly M, Wu SL, Beerhues L, Yuan SS, Hou MF, Chen SL, Chang FR, Wu YC. (2014). Bioactive 6S-styryllactone constituents of Polyalthia parviflora. Journal of Natural Products, 77, 2626-2632.
Prawat U, Chaimanee S, Butsuri A, Salae AW, Tuntiwachwuttikul P. (2012). Bioactive styryl lactone, two new naphthoquinones, and one new styryl lactone, and other constituents from Goniothalamus scortechinii. Phytochemistry Letters, 5(3), 529-534.
Ramli R, Ismail NH, Manshoor N. (2017). Recycling HPLC for the purification of oligostilbenes from Dipterocarpus semivestitus and Neobalanocarpus heimii (Dipterocarpaceae). Journal of Liquid Chromatography & Related Technologies, 40(18), 943-949.
Rasol NE, Ahmad FB, Lim XY, Chung FFL, Leong CO, Mai CW, Bihud NV, Zaki HM, Ismail NH. (2018b). Cytotoxic lactam and naphthoquinone alkaloids from roots of Goniothalamus lanceolatus Miq. Phytochemistry Letters, 24, 51-55.
Rasol NE, Ahmad FB, Mai CW, Bihud NV, Abdullah F, Awang K, Ismail NH. (2018a). Styryl lactones from roots and barks of Goniothalamus lanceolatus. Natural Product Communication, 13(12), 1575-1578.
Sidana J, Joshi LK. (2013). Recycle HPLC: a powerful tool for the purification of natural products. Chromatography Research International, 7(2), 1-7.
Talapatra B, Porel A, Biswas K, Talapatra SK. (1997). Absolute configuration of goniodiol, goniodiol monoacetate and other related dihydropyrones from synthetic, circular dichroism and X-ray crystallographic evidence. Journal of the Indian Chemical Society, 74, 896-903.
Wiart C. (2007). Goniothalamus species: a source of drugs for the treatment of cancers and bacterial infections? Evidence-Based Complementary and Alternative Medicine, 4(3), 299-311.
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Copyright (c) 2023 Seri Arisha Yaakop, Nur Vicky Bihud
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