Total Phenolic Content, Total Flavonoid Content, and Antioxidant Activity of Various Solvent Extracts from the Peels and Seeds of Marang (Artocarpus odoratissimus Blanco)
DOI:
https://doi.org/10.37134/jsml.vol13.1.10.2025Keywords:
antioxidant activity, Artocarpus odoratissimus, peels and seeds, total flavonoid content, total phenolic contentAbstract
Artocarpus odoratissimus, locally known as “marang,” is a tropical fruit native to Mindanao, Philippines. As an underutilized fruit, it remains understudied and holds significant unexplored potential for drug discovery and pharmaceutical applications. This study evaluates the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of A. odoratissimus peel and seed crude extracts obtained from using nonpolar and polar solvents. Results showed that the ethanol peel extract had the highest TPC of 129.26 mg GAE/g, while the aqueous peel extract had the highest TFC of 68.95 mg QE/g. Moreover, the aqueous peel extract exhibited very strong antioxidant activity (EC50 = 0.05 μg/mL). Overall, a general trend was observed showing that polar extracts exhibit higher TPC, higher TFC, and stronger antioxidant activities compared to the nonpolar extracts. These findings highlight the importance of selecting appropriate solvents to efficiently extract bioactive compounds, especially for maximizing antioxidant properties. Future research should investigate the aqueous peel extract, given its very strong antioxidant activity, which is more potent than that of ascorbic acid (EC50 = 6.84 μg/mL), and identify and characterize the specific bioactive compounds responsible for this potent activity.
References
Agosto NJ. (2020). Antioxidant, anti-inflammatory and anticancer potentials of three traditional medicinal plants used by the Mamanwas of Agusan and Surigao provinces. Master Thesis, Mindanao State University-Iligan Institute of Technology, Iligan City, Philippines.
Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N. (2019). Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal. Plants, 8(4), 96.
Bakar MFA, Karim FA, Perisamy E. (2015). Comparison of phytochemicals and antioxidant properties of different fruit parts of selected Artocarpus species from Sabah, Malaysia. Sains Malaysiana, 44(3), 355-363.
Bakar MFA, Mohamed M, Rahmat A, Fry J. (2009). Phytochemicals and antioxidant activity of different parts of bambangan (Mangifera pajang) and tarap (Artocarpus odoratissimus). Food Chemistry, 113(2), 479-483.
Bendary E, Francis RR, Ali HMG, Sarwat MI, Hady SE. (2013). Antioxidant and structure–activity relationships (SARs) of some phenolic and anilines compounds. Annals of Agricultural Sciences, 58(2), 173-181.
Castro CJL, Lomonsod, KC. (2021). Phytochemical profile, free radical scavenging activity and anticancer potential of Pandanus odoratissimus leaves ethanol extract. Mindanao Journal of Science and Technology, 19(2), 184-208.
de Oliveira Pateis V, Bracht L, dos Santos Castro L, Salla GBF, Comar JF, Parizotto AV, Peralta RM, Bracht A. (2018). The food additive BHA modifies energy metabolism in the perfused rat liver. Toxicology Letters, 299, 191-200.
Ekor M. (2014). The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology, 4, 177.
El Gamouz S, Bouzekri O, Elidrissi M, Amechrouq A, Choukrad M. (2022). A comparative study of phytochemical profile of different solvents’ effects on total phenolic content and antioxidant activities of various parts of Halimium halimifolium. Journal of Chemistry, 2022, 1-10.
Herrera-Pool E, Ramos-Díaz AL, Lizardi-Jiménez MA, Pech-Cohuo S, Ayora-Talavera T, Cuevas-Bernardino JC, García-Cruz U, Pacheco N. (2021). Effect of solvent polarity on the Ultrasound Assisted extraction and antioxidant activity of phenolic compounds from habanero pepper leaves (Capsicum chinense) and its identification by UPLC-PDA-ESI-MS/MS. Ultrasonics Sonochemistry, 76, 105658.
Jagtap UB, Bapat VA. (2010) Artocarpus: A review of its traditional uses, phytochemistry and pharmacology. Journal of Ethnopharmacology, 129(2), 142-166.
Jonatas KAS, Querequincia JMB, Miranda SD, Obatavwe U, Corpuz MJA, Vasquez RD. (2020). Antidiabetic evaluation of Artocarpus odoratissimus (Moraceae) fruit. Jurnal Ilmiah Farmasi, 16(1), 1-8.
Kannaian UPN, Edwin JB, Rajagopal V, Shankar SN, Srinivasan B. (2020). Phytochemical composition and antioxidant activity of coconut cotyledon. Heliyon, 6(2), e03411.
Nogata Y, Sakamoto K, Shiratsuchi H, Ishii T, Yano M, Ohta H. (2006). Flavonoid composition of fruit tissues of citrus species. Bioscience, Biotechnology, and Biochemistry, 70(1), 178-192.
Nugraha D, Yusuf AL, Wahlanto P. (2023) Narrative review: Optimation of ethanol as a solvent for flavonoid compounds in papaya leaf extraction. Ad-Dawaa Journal of Pharmacy, 1(2):107-110.
Osorio-Esquivel O, Alicia-Ortiz-Moreno N, Álvarez VB, Dorantes-Álvarez L, Giusti MM. (2011). Phenolics, betacyanins and antioxidant activity in Opuntia joconostle fruits. Food Research International, 44(7), 2160-2168.
Palaiogiannis D, Chatzimitakos T, Athanasiadis V, Bozinou E, Makris DP, Lalas SI. (2023). Successive solvent extraction of polyphenols and flavonoids from Cistus creticus L. leaves. Oxygen, 3(3), 274-286.
Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. (2017). Oxidative stress: harms and benefits for human health. Oxidative Medicine and Cellular Longevity, 2017, 416763.
Pop A, Berce C, Bolfa P, Nagy A, Catoi C, Dumitrescu I, Silaghi-Dumitrescu L, Loghin F. (2013). Evaluation of the possible endocrine disruptive effect of butylated hydroxyanisole, butylated hydroxytoluene and propyl gallate in immature female rats. Farmacia, 61(1), 202-211.
Recuenco M, De Luna JR, Magallano N, Salamane K. (2020). Phytochemical screening, total phenolics, and antioxidant and antibacterial activities of selected Philippine indigenous fruits. The Philippine Journal of Science, 149(3-a): 697-710.
Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. (2010). Oxidative stress, inflammation, and cancer: how are they linked? Free Radical Biology and Medicine, 49(11), 1603-1616.
Rufino MDSM, Alves RE, De Brito ES, Pérez-Jiménez J, Saura-Calixto F, Mancini-Filho J. (2010). Bioactive compounds and antioxidant capacities of 18 non-traditional tropical fruits from Brazil. Food Chemistry, 121(4), 996-1002.
Sharifi-Rad M, Anil Kumar NV, Zucca P, Varoni EM, Dini L, Panzarini E, Rajkovic J, Tsouh Fokou PV, Azzini E, Peluso I, Prakash Mishra A, Nigam M, El Rayess Y, Beyrouthy ME, Polito L, Iriti M, Martins N, Martorell M, Docea AO, Setzer WN, Calina D, Cho WC, Sharifi-Rad J. (2020). Lifestyle, oxidative stress, and antioxidants: back and forth in the pathophysiology of chronic diseases. Frontiers in Physiology, 11, 694.
World Health Organization. (2019). Geneva, World Health Organization; [accessed June 10, 2024]. https://www.who.int/docs/default-source/wpro---documents/countries/philippines/reports/prevention-and-control-of-noncommunicable-diseases-in-the-philippines---the-case-for-investment.pdf.
Yusof N, Munaim MSA, Kutty RV. (2020). The effects of different ethanol concentration on total phenolic and total flavonoid content in Malaysian propolis. IOP Conference Series: Material Science and Engineering, 991(1), 012033.
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