Antibacterial and Antioxidant Activities of Fruits of Solanum hirtum Vahl. from Venezuela
DOI:
https://doi.org/10.37134/jsml.vol12.1.12.2024Keywords:
Solanum, Solanum hirtum, Antibacterial activity, DPPH, Antioxidant activityAbstract
Solanum hirtum (Solanaceae) has been used in traditional medicine to heal several illnesses, it’s worked as an antiseptic for sore throat and other diseases. The present study describes the phytochemical profiles, antibacterial, and antioxidant properties of S. hirtum fruits. The phytochemicals from the dried fruits were extracted using a maceration technique with methanol and the flash column chromatography with n-hexane, dichloromethane, and methanol was the selected means to fraction the extract. The phytochemical screening was implemented using standard chemical tests. The antibacterial and antioxidant activities were screened using the disc diffusion method, and analyzing the radical scavenging activity using 2,2´-diphenyl-1-picryhydrazyl (DPPH), respectively, whilst the total phenolic content was measured using the Folin-Ciocalteu method. The phytochemical analysis results revealed the presence of terpenoids, steroids, phenols, flavonoids, and alkaloids. Antibacterial activity results showed the dichloromethane fraction exhibited activity against S. aureus (7.0 mm) and K. pneumoniae (9.0 mm) and methanolic fraction against P. aeruginosa (7.0 mm), S. aureus (8.0 mm) and K. pneumoniae (9.0 mm). In the DPPH scavenging assay, the IC50 value of the extract was 268.66±0.01 μg/mL. S. hirtum showed dose-dependent DPPH radicals scavenging activity. The total phenolic content (in gallic acid equivalents, GAE) was 18.54±0.30 mg GAE/g and the flavonoids content (in quercetin equivalents, QE) was 5.14±0.10 mg QE/g. Solanum genus is enriched with a diverse class of chemical compounds based on the literature survey and in our study, the resulting bioactivities are basically due to a mixture of phytochemicals possessed in the extracts or may be due to the synergistic activity of various constituents held by this plant species.
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References
Akar Z, Kuçuk M, Dogan H. (2017). A new colorimetric DPPH scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 640-647.
Anosike CA, Obidoa O, Ezeanyika LU. (2012). Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). DARU Journal of Pharmaceutical Sciences, 20, 1-7.
Banu KS, Cathrine L. (2015). General techniques involved in phytochemical analysis. International Journal of Advanced Research in Chemical Science, 2(4), 25-32.
Benítez de Rojas CE. (1994). Reseña crítica del estudio de la familia Solanaceae en Venezuela. In: Proceeding of the VI Congreso Latinoamericano de Botánica. (Fortunato, R. & N. Bacigalupo, eds.). Monographs in Systematic Botany from the Missouri Botanical Garden, 68, 3-15.
Bewick V, Cheek L, Ball J. (2004). Statistics review 9: one-way analysis of variance. Critical Care, 8, 130-136.
CLSI. 2017. Performance Standards for Antimicrobial Susceptibility Testing. 27th ed. CLSI, United State.
Coletto A, Kinupp V, Absy M, Kerr W. (2004). Pollen morphology and study of the visittors (Hymenoptera, Apidae) in central Amazoniam. Acta Botánica Brasilica, 18, 653-657.
Diggle PK. (1991). Labile sex expression in andromonoecious Solanum hirtum: floral development and sex determination. American Journal of Botany, 78(3), 377-393
Diggle PK. (1993). Developmental plasticity, genetic variation, and the evolution of andromonoecy in Solanum hirtum (Solanaceae). American Journal of Botany, 80(8), 967-973.
Doss A, Mubarack HM, Dhanabalan R. (2009). Antibacterial activity of tannins from the leaves of Solanum trilobatum Linn. Indian Journal Science Technology, 2(2), 41-43.
Elizalde-Romero CA, Montoya-Inzunza LA, Contreras-Angulo LA, Heredia JB, Gutiérrez-Grijalva EP. (2021). Solanum fruits: phytochemicals, bioaccessibility and bioavailability, and their relationship with their health-promoting effects. Frontiers in Nutrition, 8, 790582.
El-Sherbini GT, Zayed RA, El-Sherbini ET. (2009). Molluscicidal activity of some Solanum species extracts against the snail Biomphalaria alexandrina. Journal of Parasitology Research, 474360.
Fekry MI, Ezzat SM, Salama MM, Alshehri OY, Al-Abd AM. (2019). Bioactive glycoalkaloides isolated from Solanum melongena fruit peels with potential anticancer properties against hepatocellular carcinoma cells. Scientific Reports, 9(1), 1746.
González M, Zamilpa A, Marquina S, Navarro V, Álvarez L. (2004). Antimycotic spirostanol saponins from Solanum hispidum leaves and their structure− activity relationships. Journal of Natural Products, 67(6), 938-941.
Ibarrola DA, Arrua W, González JE, Escobar MS, Centurión J, Benítez AC, Hellión-Ibarrola MC. (2022). The antihypertensive and diuretic effect of crude root extract and saponins from Solanum sisymbriifolium Lam., in L-NAME-induced hypertension in rats. Journal of Ethnopharmacology, 298, 115605.
Ikeda T, Tsumagari H, Honbu T, Nohara T. (2003). Cytotoxic activity of steroidal glycosides from Solanum plants. Biological and Pharmaceutical Bulletin, 26(8), 1198-1201.
Kato S, Aoshima H, Saitoh Y, Miwa N. (2009). Highly hydroxylated or γ-cyclodextrin-bicapped water-soluble derivative of fullerene: The antioxidant ability assessed by electron spin resonance method and β-carotene bleaching assay. Bioorganic & Medicinal Chemistry Letters, 19(18), 5293-5296.
Kaunda JS, Zhang YJ. (2019). The Genus Solanum: An Ethnopharmacological, Phytochemical and biological properties review. Natural Products and Bioprospecting, 9(2), 77-137.
Mohan M, Attarde D, Momin R, Kasture S. (2013). Antidepressant, anxiolytic and adaptogenic activity of torvanol A: an isoflavonoid from seeds of Solanum torvum. Natural Product Research, 27(22), 2140-2143.
Muruhan S, Selvaraj S, Viswanathan PK. (2013). In vitro antioxidant activities of Solanum surattense leaf extract. Asian Pacific Journal of Tropical Biomedicine, 3(1):28-34.
Okwu DE. (2001). Evaluation of chemical composition of medicinal plants belonging to Euphorbiaceae. Pakistan Veterinary Journal, 14(1), 160-162.
Rojas L, Jaramillo C, Lemus M. (2015). Métodos analíticos para la determinación de metabolitos secundarios de plantas. Universidad Machala, Ecuador. pp.108.
Salinas P. (2012). Plantas tóxicas comunes en el estado Mérida, Venezuela. 3ª parte Revista de Facultad de Medicina, Universidad de Los Andes, 21, 93-104.
Sofowra A. (1993). Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd., Ibadan, Nigeria, pp. 289.
Sulekha M, Satish Y, Sunita Y, Nema RK. (2009). Antioxidants: a review. Journal of Chemical and Pharmaceutical Research, 1(1), 102-104.
Thirumurugan D, Cholarajan A, Raja SS, Vijayakumar R. (2018). An Introductory Chapter: Secondary Metabolites. InTech.
Twaij BM, Hasan MN. (2022). Bioactive secondary metabolites from plant sources: types, synthesis, and their therapeutic uses. International Journal of Plant Biology, 13, 4-14.
Velasco J, Rojas J, Salazar P, Rodríguez M, Díaz T, Morales A, Rondón M. (2007). Antibacterial activity of the essential oil of Lippia oreganoides against multiresistant bacterial strains of nosocomial origin. Natural Product Communication, 2(1), 85-88.
Villano D, Fernández-Pachon MS, Moya ML, Troncoso AM, García-Parrilla MC. (2007). Radical scavenging ability of polyphenolic compounds towards DPPH free radical. Talanta, 71(1), 230-235.
Yousafa Z, Wanga Y, Baydounc E. (2013). Phytochemistry and pharmacological studies on Solanum torvum Swartz. Journal of Applied Pharmaceutical Science, 3(4), 152-160.
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Copyright (c) 2024 Rubén Ramirez, Alida Pérez-Colmenares, Ysbelia Obregón-Díaz, Luis Rojas-Fermín, Yndra Cordero, Wilberto De Lima
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