Phytochemical study of stem bark from Alstonia spathulata Bl. (Apocynaceae) (8 - 15)
Keywords:
Phytochemical, Alstonia spathulata, bark extracts, hexane, chromatography, 1D-NMR, 2D-NMRAbstract
In Malaysia, Alstonia spathulata Bl. is locally known as ‘Pulai Basong’ which distributein peat forest. The separation of the chemical components from hexane and DCM crudeextract of Alstonia spathulata was carried out using column chromatography (CC) andthin layer chromatography (TCL) techniques. A total of five known compound wereisolated from the stem bark extracts and identified as: β-amyrin, β-amyrin acetate, lupeolacetate, stigmasterol and β-sitosterol. The elucidation structures of these compounds wereestablished using 1D-NMR (1H, 13C and DEPT) and 2D-NMR (COSY, HSQC, HMBC)spectroscopic analysis, involving also comparison with data from the literature.