Unveiling the Phytochemical Richness of Guatemalan Spondias: Comparative Analysis of Phenolics, Flavonoids and Antioxidant Potential
DOI:
https://doi.org/10.37134/jsml.vol13.2.13.2025Keywords:
polyphenols, p-coumaric acid, rutin, quercetin, nutraceutical, Spondias purpurea, Spondias mombinAbstract
Jocote fruits, derived from Spondias purpurea and Spondias mombin, are widely consumed in Guatemala and are traditionally valued for their nutritional and medicinal properties. Previous studies conducted in other regions highlight their richness in vitamins and phenolic constituents with potential health benefits. This study evaluated the flavonoid composition, total phenolic content, and antioxidant capacity of nine varieties of the two Spondias species collected from distinct regions of Guatemala to assess their potential as nutraceutical resources. Antioxidant activity was determined using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, while total phenolics were quantified by the Folin–Ciocalteu method. Flavonoids and related phenolic compounds were profiled using HPLC-DAD, with compound identification based on retention time comparison with analytical standards. Extracts from the Corona, Tronador, and Rax Ux varieties of S. purpurea and the Jobo and Quinin varieties of S. mombin exhibited the strongest antioxidant activity, with IC₅₀ values ranging from 6.19 to 7.96 mg/L. Across the samples, p-coumaric acid and rutin were the most frequently detected phytochemicals (present in 14 samples), followed by quercetin (detected in 13 of the 15 samples). These findings demonstrate that Guatemalan jocote fruits possess a variety of bioactive flavonoids and phenolic compounds, supporting their classification as nutraceutical candidates due to their strong antioxidant potential. Further pharmacological and cytotoxicity studies are recommended to elucidate additional health-related benefits and broaden their potential applications in nutraceutical development.
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References
Adorno L, Batista L, Reis E, Peruch G, Branc A. (2020). Characterization of a flavonol-rich antioxidant fraction from Spondias purpurea L. pulp and the effect of its incorporation on cellulose acetate-based film. Journal of the Science of Food and Agriculture, 101(8), 3270-3279. doi:10.1002/jsfa.10956.
Araújo A, Maia S, Mendes M, Ferreira D, Costa C, de Almeida R, Pinto Í, Florindo M. (2012). Chemical composition, antioxidant and antibacterial activities of two Spondias species from Northeastern Brazil. Journal of Pharmaceutical Biology, 50(6), 740-746. doi:10.3109/13880209.2011.627347
Boz H. (2015). p-Coumaric acid in cereals: presence, antioxidant and antimicrobial effects. International Journal of Food Science and Technology, 50, 2323-2328. doi:10.1111/ijfs.12898
de Freitas JS, Novo AA, Kunigami CN, Moreira DL, Freitas SP, da Matta VM, Jung EP, Ribeiro LO. (2024). Spondias tuberosa and Spondias mombin: Nutritional composition, bioactive compounds, biological activity and technological applications. Resources, 13, 68. doi:10.3390/resources13050068
Dias MC, Pinto DCGA, Silva MS. (2021). Plant flavonoids: Chemical characteristics and biological activity. Molecules, 26, 5377. doi:10.3390/molecules26175377
dos Santos ÉM, Ataide JA, Coco JC, Fava ALM, Silvério LAL, Sueiro AC, Silva JRA, Lopes AM, Paiva-Santos AC, Mazzola PG. (2023). Spondias sp: Shedding light on its vast pharmaceutical potential. Molecules, 28(4), 1862. doi:10.3390/molecules28041862
Elufioye TO, Berida T I (2018). GC-MS analysis and antioxidant activity of Spondias purpurea L (Anacardiaceae). Pharmacognosy Journal, 10(5), 941-945. doi:10.5530/pj.2018.5.159
Engels C, Gräter D, Esquivel P, Jiménez V, Gänzle M, Schieber A. (2012). Characterization of phenolic compounds in jocote (Spondias purpurea L.) peels by ultra-high-performance liquid chromatography/electrospray ionization mass spectrometry. Journal of Food Research International, 46, 557-562. doi:10.1016/j.foodres.2011.04.003
Escamilla C, Cuevas E, Guevara J. (2009). Flavonoides y sus acciones antioxidantes. Revista de la Facultad de Medicina, 2, 73-75.
Hasnat H, Shompa SA, Islam MM, Alam S, Richib FT, Emon NU, Ashrafi S, Ahmed NU, Chowdhurye MNR, Fatema N, Hossain MS, Ghosh A, Ahmed A. (2024). Flavonoids: A treasure house of prospective pharmacological potentials. Heliyon, 10, e27533. doi:10.1016/j.heliyon.2024.e27533
Iriti M, Kubina R, Cochis A, Sorrentino R, Varoni E, Kabala-Dzik A, Azzimonti B, Dziedzic A, Rimondini L, Wojtyczka R. (2017). Rutin, a quercetin glycoside, restores chemosensitivity in human breast cancer cells. Phytotherapy Research, 31(10), 1529-1538. doi:10.1002/ptr.5878
Jaganathan S, Supriyanto E, Mandal M. (2013). Events associated with apoptotic effect of p-coumaric acid in HCT-15 colon cancer cells. World Journal of Gastroenterology, 19, 7726-7734. doi:10.3748/wjg.v19.i43.7726
Kadir NHA, Salleh WMNHW, Ghani NA, Rasol NE, Khamis S, Juhari MAA. (2023). Flavonoids from Syzygium pyrifolium. Chemistry of Natural Compounds, 59(5), 929-931. doi:10.1007/s10600-023-041-52-y
Lasota M, Lechwar P, Kukula-Koch W, Gaweł K, Czop M. (2024). Pulp or Peel? Comparative analysis of the phytochemical content and selected cosmetic-related properties of Annona cherimola L., Diospyros kaki Thumb., Cydonia oblonga Mill. and Fortunella margarita Swingle pulp and peel extracts. Molecules, 29, 1133. doi:10.3390/molecules29051133
Li Y, Guo C, Yang J, Wei J, Xu J, & Cheng, S. (2006). Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chemistry, 96, 254-260. doi:10.1016/j.foodchem.2005.02.033
Magiera S, Zareba M. (2015). Chromatographic determination of phenolic acids and flavonoids in Lycium barbarum L. and evaluation of antioxidant activity. Journal of Food Analytical Methods, 8, 2665-2674. doi:10.1007/s12161-015-0166-y
Martínez S, González J, Culebras J, Tuñón M. (2002). Los flavonoides: propiedades y acciones antioxidantes. Nutrición Hospitalaria, 17(6), 271-278.
Miller A, Schaal B. (2005). Domestication of a Mesoamerican cultivated fruit tree, Spondias purpurea. Proceedings of the National Academy of Sciences of the United States of America, 102(36), 12801-12806. doi:10.1073/pnas.0505447102
Ogunro OB, Oyeyinka BO, Batiha GES. (2023). Nutritional benefits, ethnomedicinal uses, phytochemistry, pharmacological properties and toxicity of Spondias mombin Linn: A comprehensive review. Journal of Pharmacy and Pharmacology, 75(2), 162-226. doi:10.1093/jpp/rgac086
Ordaz-Trinidad N, Dorantes-Alvarez L, Salas-Benito J, Barrón-Romero B, Salas-Benito M, de Nova-Ocampo M. (2018). Citotoxicidad y actividad antiviral de extractos de chiles (Capsicum spp). Revista Polibotánica, 46, 273-285. doi:10.18387/polibotanica.46.18
Pérez G, Martínez G. (2001). Los Flavonoides como antioxidantes naturales. Acta Farmacéutica Bonaerense, 20(4), 297-306.
Pérez Sabino JF, Farfán Barrera C, Oliva Hernández BE, Jayes Reyes PG, Mérida Reyes MS, Muñoz Wug M. (2012). Determinación de los flavonoides en seis plantas del género Lippia (Verbenaceae) nativas de Guatemala como posibles fuentes de nutracéuticos (Informe final). Dirección General de Investigación, Universidad de San Carlos de Guatemala. Guatemala.
Pragasam S, Venkatesan V, Rasool, M. (2012). Immunomodulatory and anti-inflammatory effect of p-coumaric acid, a common dietary polyphenol on experimental inflammation in rats. Inflammation, 36(1), 169-176. doi:10.1007/s10753-012-9532-8
Ribeiro LO, de Freitas BP, Lorentino CMA, Frota HF, dos Santos ALS, Moreira DL, do Amaral DS, Jung EP, Kunigami CM. (2022). Umbu fruit peel as source of antioxidant, antimicrobial and-amylase inhibitor compounds. Molecules, 27, 410. doi:10.3390/molecules27020410
Rodrigues FAM, Guedes JAC, Giffony PS, dos Santos SBF, Ribeiro MENP, da Silva KMR, Zocolo GJ, de Araújo TG, Ricardo NMPS. (2021). Spondias purpurea L. stem bark extract: Antioxidant and in vitro photoprotective activities. Journal of the Brazilian Chemical Society, 32(10), 1918-1930. doi:10.21577/0103-5053.20210082
Rodrigues NL, da Costa Souza AL, Cunha de Oliveira C, Garcez de Carvalho M, Romao de Lima B, de Almeida Aksutu RCC, Silva de Lana V, Raposo A, Saraiva A, Han H., Motezano de Carvalho IM. (2024). Nutritional and biological attributes of Spondias tuberosa (Umbu) fruit: An integrative review with a systematic approach. Journal of Food Composition and Analysis, 130, 106196. doi:10.1016/j.jfca.2024.106196
Salihu AS, Salleh WMNHW. (2023). Flavonoids and other constituents of plants from the genus Knema (Myristicaceae) and their pharmacological studies: An updated review. Vietnam Journal of Chemistry, 61(4), 397-411. doi:10.1002/vjch.202200224
Salleh WMNHW, Ahmad F, Khong HY, Zulkifli RM, Sarker SD. (2016). Madangones A and B: Two new neolignans from the stem bark of Beilschmiedia madang and their bioactivities. Phytochemistry Letters, 15, 68-173. doi:10.1016/j.phytol.2016.01.004
Salleh WMNHW, Ahmad F, Khong HY. (2014). Antioxidant and anti-tyrosinase activities from Piper officinarum C.DC (Piperaceae). Journal of Applied Pharmaceutical Sciences, 4(5), 87-91. doi:10.7324/JAPS.2014.40516
Salleh WMNHW, Ahmad F, Khong HY. (2015). Evaluation of antioxidant, anticholinesterase and antityrosinase activities of five Cinnamomum species. Dhaka University Journal of Pharmaceutical Sciences, 14(2), 125-132. doi:10.3329/dujps.v14i2.28500
Salleh WMNHW, Ahmad F. (2016). Antioxidant and anticholinesterase activities of essential oil of Alseodaphne peduncularis Meisn. (Lauraceae). Turkish Journal of Pharmaceutical Science, 13(3), 347-350. doi:10.4274/tjps.2016.11
Sameh S, Al-Sayed E, Labib RM, Singab AN. (2018). Genus Spondias: A phytochemical and pharmacological review. Evidence-Based Complementary and Alternative Medicine, 5382904. doi:10.1155/2018/5382904
Sharma OP, Bhat TK. (2008). DPPH antioxidant assay revisited. Food Chemistry, 113, 1202-1205. doi:10.1016/j.foodchem.2008.08.008
Tarahovsky Y, Kim Y, Yagolnik E, Muzafarov E. (2014). Flavonoid-membrane interactions: Involvement of flavonoid-metal complexes in raft signaling. Journal of Biochimica et Biophysica Acta, 1838, 1235-1246. doi:10.1016/j.bbamem.2014.01.021
Turatbekova A, Babamuradova L, Tasheva U, Saparbaeva N, Saibnazarova G, Turayeva M, Yakubov Y. (2023). A brief review on biological and chemical activities of flavonoids in plants. E3S Web of Conferences, 434, 03026. doi:10.1051/e3sconf/202343403026
Vasco C, Ruales J, Kamal-Eldin A. (2008). Total phenolic compounds and antioxidant capacities of major fruits from Ecuador. Food Chemistry, 111, 816-823. doi:10.1016/j.foodchem.2008.04.054
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Copyright (c) 2025 Pedro Pablo Molina-Jauregui, Max Samuel Mérida-Reyes, Antonio Jorge Ribeiro da Silva, Juan Francisco Perez Sabino
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