The Detection of Phytochemical Properties from Freeze Dried Ottochloa nodosa (Kunth) Dandy Extracts and Its Potential as a Bacterial Inhibitor
Keywords:Ottochloa nodosa (Kunth) Dandy, secondary metabolites, bacterial inhibitor, freeze dried, zone inhibition
This study aimed to detect the secondary metabolites and chemical components in the freeze-dried extract of Ottochloa nodosa (Kunth) Dandy. In addition, discovering the extract could have potential as a bacterial inhibitor by determination of the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) carried out in extracts. Determination of antimicrobial potential of freeze-dried extracts was conducted through phytochemical screening, gas chromatography-mass spectrometry (GC-MS) test, agar disc diffusion and broth dilution methods. The study shows this extract has bacterial inhibitors’ potential since it contained phytochemical compounds such as alkaloid, saponin, flavonoid and tannin, while 9 chemical components have been displayed through GC-MS analysis. The disc diffusion method was done at different concentrations, at 25, 50 and 100 mg/ml. The result also shows that the extracts are able to inhibit the gram-positive and gram-negative bacteria. Freeze-dried extract presented significant differences in 100 mg/ml and 50 mg/ml, p=0.05. The MIC value was found at 2.0 mg/ml in the agar plate containing methicillin-resistant S. aureus (MRSA), Aeromonas hydrophila and Staphylococcus aureus, while Vibrio parahaemolyticus was at 5.0 mg/ml. While the MBC value showed 2.0 mg/ml in MRSA and S. aureus (since the plates containing A. hydrophila and V. parahaemolyticus had bacterial growths, thus, it demonstrated that the plant extracts were only able to inhibit the bacterial growth but could not kill them). In conclusion, the freeze-dried method can be applied for water removal during plant extraction and possess bacterial inhibition activity. This study implicated that O. nodosa extract is a natural source of antibiotic substances for bacterial infection treatment.
Abdullah, S., Shaari, A.R. & Azimi, A. (2012). Effect of drying methods on metabolites composition of misai kucing (Orthosiponstamineus) leaves. APCBEE Procedia, 2, 178-182.
Alabi, O.A, Haruna, M.T., Anokwuru, C.P., Jegede, T., Abia, H., Okegbe, V.U., & Ehsan, B.E. (2012). Comparative studies on antimicrobial properties of extracts of fresh and dried leaves of Carica papaya (L) on clinical bacterial and fungal isolates. Advances in Applied Science Research, 3(5), 3107-3114.
Al-Momami, W., Abu-Basha, E., Janakat, S., Nicholas, R.A. & Ayling, R.D. (2007). In vitro anti mycoplasmal activity of six Jordanian medicinal plants against three Mycoplasma species. Tropical Animal Health and Production, 39, 515-519.
Amor, I.L.B., Boubaker, J., Sgaier, M.B., Skandrani, I. & Bhouri, W. (2009). Phytochemistry and biological activities of Phlomis species. Journal of Ethnopharmacology, 125,183-202.
Ashour, A.S., El Aziz M.M.A, & Melad, A.S.G. (2019). A review on saponins from medicinal plants: chemistry, isolation, and determination. Journal of Nanomedicine Research, 8(1), 282-288.
Azwanida, N.N. (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants Research, 4, 196.
Bais, H.P., Walker, T.S., Schweizer, H.P. & Vivanco, J.M. (2002). Plant Physiology and Biochemistry, 40, 983-995.
Bisignano, G., Sanogo, R., Marino, A., Aquino, R., D'Angelo, V., Germanò, M.P., De Pasquale, R., & Pizza C. (2000). Antimicrobial activity of Mitracarpus scaber extract and isolated constituents. Letters in Applied Microbiology, 30, 105-108.
Bouzada, M.L.M., Fabri, R.L., Nogueira, M, Konno, T.U.P., Duarte, G.G., & Scio, E. (2009). Antibacterial, cytotoxic and phytochemical screening of some traditional medicinal plants in Brazil. Pharmaceutical Biology, 47, 44-52.
Chakraborty, A. & Brantner, A.H. (1999). Antibacterial steroid alkaloids from the stem bark of Holarrhena pubescens. Journal of Ethnopharmacology, 68, 339-344.
Compean, K.L. & Ynalvez, R.A. (2014). Antimicrobial activity of plant secondary metabolites: A review. Research Journal of Medicinal Plants, 8(5), 204-213.
Cosa, P., Vlietinck, A.J., Berghe D.V., Maes, L. (2006). Anti-infective potential of natural products. How to develop a stronger in vitro ‘proof-of-concept’. Journal of Ethnopharmacology, 106, 290-302.
Cowan, M.M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564-582.
Cushine, T.P.T. & Lamb, A.J. (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26, 343-356.
Dai, J. & Mumper, R.J. (2010). Plant phenolics: extraction, analysis and their antioxidant and anti-cancer properties. Molecules, 15, 7313-7352.
Dixon, R.A., Dey, P.M. & Lamb, C.J. (1983). Phytoalexins: enzymology and molecular biology. Advances in Enzymology, 55, 1-69.
Faustino, M.A.F., Faustino, M.A.F., & Pinto, C.G.A. (2019). Halophytic grasses, a new source of nutraceutical? A review on their secondary metabolites and biological activities. International Journal of Molecular Sciences, 20, 1-30.
Goerch, M.C.D.S., Schafer, L., Tonial, M., Oliveira, V.R.D., Ferraz, A.B.D.F., Fachini, J., Silva, J.B.D., & Harborne, J. (1973). Phytochemical methods: a guide to modern techniques of plant analysis. Chapman & Hall, London.
Hatab, S., Athanasio, R., Holley, R., Rodas-Gonzalez, A., & Narvaez-Bravo, C. (2016). Survival and reduction of shiga toxin-producing E. coli in a fresh cold-pressed juice treated with antimicrobial plant extracts. Journal of Food Science, 81, 1987-1995.
Johnson, D.B., Shringi, B.N., Kumar, D., Sai, N. & Chalichem, S. (2011). Screening of antimicrobial activity of alcoholic & aqueous extract of some indigenous plants. Journal of Pharmaceutical Sciences, 1(2), 186-193.
Karlina, C.Y., Ibrahim, M. & Trimulyono, G. (2013). Aktivitas antibakteri ekstrak herba krokot (Portulaca oleracea L.) terhadap Staphylococcus aureus dan Escherichia coli. E- Journal Unesa LenteraBio, 2, 87-93.
Lalitha, M.K. (2004). Manual on antimicrobial susceptibility testing. American Society for Microbiology, USA.
Liu, Y., Zhao, Y., & Feng, X. (2008). Exergy analysis for a freeze-drying process. Applied Thermal Engineering, 28, 675-690.
Manidool, C. (1989). Natural grassland and native grasses of Thailand. Technical bulletin no 1301-26-32.
Manilal, A., Sujith, S., Sengah, S., Selvin, S.K., & Shakir, C. (2009). Cytotoxic potentials of Red Alga, Laurencia brandenii collected from the Indian Coast. Global Journal of Pharmacology, 3(2), 90-94.
Mello, V.J., Gomes, M.T, Lemos, F.O., Delfino, J.L, Andrade, S.P., Lopes, M.T., & Salas, C.E. (2008). The gastric ulcer protective and healing role of cysteine proteinases from Carica candamarcensis. Phytomedicine, 15, 237-244.
Morrone, O., Vega, A.S., & Maier, M. (2000). Elaiosomes in Urochloa paucispicata (Poaceae): anatomy and chemical composition. Flora, 195, 303-310.
Ndip, R.N., Malange, A.E., Tarkang, A.E., Mbullah, S.M., Luma, H.N., Malongue, A., Ndip, L.M., Nyongbela, K., Wirmum, C., & Efange, S.M. (2007). In vitro anti Helicobacter pylori activity of extracts of selected medicinal plants from North West Cameroon. Journal of Ethnopharmacology, 4, 452-457.
Nepal, P., Singh, M., Baniya, A., Singh, S., Sainju, H.K., Shresta, R. (2018). Comparative antioxidant, antimicrobial, and phytochemical assessments of leaves of Desmostachya bipinnata L. Stapf, Hordeum vulgare L. and Drepanostachyum falcatum (Nees) Keng f. Nepal Journal of Biotechnology, 6(1),1-10.
NIH. (2009). Understanding microbes in sickness and in health. National Institute of Allergy and Infectious Disease, U.S. department of health and human services. NIH publication no.09-4914.
Prochnow, A.M., Maryse, C., Hong, J. & Murphy, A.B. (2016). Gram positive and gram- negative bacteria differ in their sensitivity to cold plasma. Scientific Reports, 6, 38610.
Rakhimov, R.R., Benetis, N.P., Lund, A., Hwang, J.S., Prokofer, A.I., & Lebeder, Y.S. (1996). Intramolecular and reorientation dynamics of bis(triphenylphosphine)-3,6-di-tert-butyl-4,5-dimethoxy-o-semiquinone complex of copper (I) in viscous media. Chemical Physics Letters, 225, 156-162.
Robert, M.F. & Wink, M. (1998). Alkaloids: biochemistry, ecology and medicinal applications. New York, Springer.
Rollins, D.M., Temenak, J.J., Shields, P., & Joseph, S.W. (2003). Microbial pathogenesis laboratory manual, 2nd Edition.
Samiha, M., Dickinson, J.A., & Somayaji, R. (2020). Update on the adverse effects of antimicrobials therapies in community practice. Mohsen, S., Dickinson, J. A., & Somayaji, R. (2020). Update on the adverse effects of antimicrobial therapies in community practice. Canadian Family Physician Medecin de Famille Canadien, 66(9), 651-659.
Santos, C.C., Guiné, R.P., & Barros, A. (2014). Effect of drying temperature on the phenolic composition and antioxidant activity of pears of Rocha variety (Pyrus Communis L.). Journal of Food Measurement and Characterization, 8(2), 105-112.
Schito, G.C. (2006). The importance of the development of antibiotic resistance in S. aureus. Clinical Microbiology and Infection, 1, 3-8.
Setzer, W.N., Setzer, M.C., Bates, R.B., & Jakes, B.R. (2000). Biologically active triterpenoid of Syncarpia glomulifera bark extract from Paluma, North Queensland, Australia. Planta Medica, 66, 176-177.
Desa, S., Khalaf, A.S.A., Baharum, S.N., & Mudhafar, M. (2020). Identification of bioactive compounds from seeds oil of agricultural waste in Malaysia. International Journal of Pharmaceutical Sciences Review and Research, 13, 1-8.
Shekhar, S. & Petersen, F. C. (2020). The dark side of antibiotics: adverse effects on the infant immune defense against infection. Frontiers in Pediatrics, 8, 54440.
Sofowara, A. (1993). Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd, Ibadan, Nigeria.
Sohail, M.N., Karim, A., Sarwar, M., & Alhasin, A.M. (2011). Onion (Allium cepa L): An alternate medicine for Pakistani population. International Journal of Pharmacology, 7, 736-744.
Takashi, O., Cai, Z., Toda, M., Hara, Y., & Shimamura, T. (1995). Appearance of antibacterial activity of oxacillin against MRSA in the presence of catechin. Kansenshogaku Zasshi, 69, 1126-b4.
Tanaka, O., Ikeda, T., Ohtani, K., Kasai, R., & Yamasaki, K. (2000). Antiyeast steroidal saponins from Yucca Schidigeria (Mohave Yucca), a new anti-food-deteriorating agent. Journal of Natural Products, 63, 332-338.
Trease, G.E. & Evans, W.C. (1989a). A textbook of pharmacognosy. Bailliere Tindall Ltd, London.
Vaghasiya, Y., Dave, R. & Chanda, S. (2011). Phytochemical analysis of some medicinal plants from Western region of India, Research Journal of Medicinal Plants, 5, 567-576.
Venugopal, R. & Liu, R.H. (2012). Phytochemicals in diets for breast cancer prevention; the importance of resveratrol and ursolic acid. Food Science and Human Wellnesslness, 1, 1-13.