Phytochemicals and Antibacterial Activity of Zingiber zerumbet Growing in Negeri Sembilan, Malaysia

Authors

  • Nur Atielia Preshahdin Faculty of Applied Sciences, Universiti Teknologi MARA, Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000 Kuala Pilah, Negeri Sembilan, Malaysia
  • Nor Akmalazura Jani Faculty of Applied Sciences, Universiti Teknologi MARA, Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000 Kuala Pilah, Negeri Sembilan, Malaysia
  • Rashidah Iberahim Faculty of Applied Sciences, Universiti Teknologi MARA, Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000 Kuala Pilah, Negeri Sembilan, Malaysia

DOI:

https://doi.org/10.37134/jsml.vol11.1.3.2023

Keywords:

Zingiber zerumbet, Zingiberaceae, essential oil, phytochemicals, antibacterial

Abstract

The present study is aimed at analysing the chemical composition of essential oil and screening the phytochemicals in crude extracts from the rhizomes of Zingiber zerumbet (L.) Roscoe ex Sm. (Zingiberaceae) as well as determining their antibacterial activity. The essential oil was extracted from the fresh rhizomes using the hydrodistillation method and analysed by gas chromatography-mass spectrometry (GC-MS). The phytochemicals from the dried rhizomes were extracted by sequential maceration technique using n-hexane, ethyl acetate, and methanol, while phytochemical screening was carried out using standard chemical tests. Antibacterial activity was screened using the disc diffusion method. A total of 31 components (94.5%) were detected in the rhizome oil with zerumbone (28.4%), terpinen-4-ol (16.5%) and α-humulene (11.1%) as the main components. Phytochemical screening of the extracts obtained from the dried rhizomes disclosed the existence of steroids, terpenes, quinones, flavonoids, phenols, alkaloids, tannins, coumarins and glycosides. The rhizome oil showed potent antibacterial activity towards Salmonella typhi and Escherichia coli with inhibition zone diameters of 20.66 and 16.66 mm, respectively. Among the extracts, the methanol extract demonstrated high inhibitory activity against Bacillus cereus (14.00 mm), Staphylococcus aureus (9.00 mm), and Salmonella typhi (10.66 mm), while the ethyl acetate extract gave the biggest inhibition zone on E. coli (10.33 mm). The findings from this study indicate that Z. zerumbet might be one of a promising source of natural chemicals that can act as antibacterial agents.

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References

Abarca-vargas R, Peña Malacara CF, Petricevich VL. (2016). Characterization of chemical compounds with antioxidant and cytotoxic activities in Bougainvillea x Buttiana Holttum and Standl (var. Rose) extracts. Antioxidants, 5, 45.

Alemu A, Tamiru W, Nedi T, Shibeshi W. (2018). Analgesic and anti-inflammatory effects of 80% methanol extract of Leonotis ocymifolia Iwarsson leaves in rodent models. Evidence-Based Complementary and Alternative Medicine, 2, 1-8.

Ali S, Khan MR, Irfanullah SM, Zahra Z. (2018). Phytochemical investigation and antimicrobial appraisal of Parrotiopsis jacquemontiana (Decne) Rehder. BMC Complementary and Alternative Medicine, 18, 43.

Anulika NP, Ignatius EO, Raymond ES, Osasere OI, Abiola AH. (2016). The chemistry of natural product: Plant secondary metabolites. International Journal of Technology Enhancements and Emerging Engineering Research, 4(8), 1-8.

Arruda M, Viana H, Rainha N, Neng NR, Rosa JS, Nogueira JM, Barreto MDC. (2012). Anti-acetylcholinesterase and antioxidant activity of essential oils from Hedychium gardnerianum Sheppard ex Ker-Gawl. Molecules, 17, 3082-3092.

Ayeni EA, Abubakar A, Ibrahim G, Atinga V, Muhammad Z. (2018). Phytochemical, nutraceutical and antioxidant studies of the aerial parts of Daucus carota L. (Apiaceae). Journal of Herbmed Pharmacology, 7(2), 68-73.

Azelan NA, Hasham R, Awang MA, Malek RA, Musa NF, Aziz RA. (2015). Antibacterial activity Zingiber officinale and Zingiber zerumbet essential oils extracted by using turbo extractor. Jurnal Teknologi, 77(3), 43-47.

Baby S, Dan M, Thaha ARM, Johnson AJ, Kurup R, Balakrishnapillai P, Lim CK. (2009). High content of zerumbone in volatile oils of Zingiber zerumbet from southern India and Malaysia. Flavour and Fragrance Journal, 24, 301-308.

Bajpai VK, Rahman A, Shukla S, Mehta A, Shukla S, Yassir Arafat SM, Rahman MM, Ferdousi Z. (2009). Antibacterial activity of leaf extracts of Pongamia pinnata from India. Pharmaceutical Biology, 47(12), 1162-1167.

Batubara I, Suparto IH, Sadiah S, Matsuoka R, Mitsunaga T. (2013). Effect of Zingiber zerumbet essential oils and zerumbone inhalation on body weight of Sprague Dawley rat. Pakistan Journal of Biological Sciences. 16(19), 1028-1033.

Benmehdi H, Hasnaoui O, Benali O, Salhi F. (2012). Phytochemical investigation of leaves and fruits extracts of Chamaerops humilis L. Journal of Materials and Environment Science, 3(2), 320-237.

Bhuiyan MNI, Chowdhury JU, Begum J. (2009). Chemical investigation of the leaf and rhizome essential oils of Zingiber zerumbet (L.) Smith from Bangladesh. Bangladesh Journal of Pharmacology, 4, 9-12.

Chane-Ming J, Vera R, Chalchat, J.C (2003). Chemical composition of the essential oil from rhizomes, leaves and flowers of Zingiber zerumbet Smith from Reunion Island. Journal of Essential Oil Research, 15, 202–205.

Chien TY, Chen LG, Lee CJ, Lee FY, Wang CC. (2008). Anti-inflammatory constituents of Zingiber zerumbet. Food Chemistry, 110, 584-589.

Chowdhury SP, Banik A, Aurin SA, Saima S. (2019). Comparison of individual and synergistic antimicrobial activity of common spices against certain infectious pathogen in Bangladesh. American Journal of Plant Sciences, 10, 1599-1611.

Fadli M, Saad A, Sayadi S, Chevalier J, Mezrioui NE, Pagès JM, Hassani L. (2012). Antibacterial activity of Thymus maroccanus and Thymus broussonetii essential oils against nosocomial infection-bacteria and their synergistic potential with antibiotics. Phytomedicine, 19, 464-471.

Fejér J, Gruľová D, Eliašová A, Kronb I, De Feo V. (2018). Influence of environmental factors on content and composition of essential oil from common juniper ripe berry cones (Juniperus communis L.). Plant Biosystems, 152, 1227-1235.

Gowri SS, Vasantha K. (2010). Phytochemical screening and antibacterial activity of Syzygium cumini (L.) (Myrtaceae) leaves extracts. International Journal of PharmTech Research, 2(2), 1569–1573.

Gul R, Jan SU, Faridullah S, Sherani S, Jahan N. (2017). Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from Ephedra intermedia indigenous to Balochistan. The Scientific World Journal, 1, 1-7.

Iqbal E, Salim KA. Lim LBL. (2015). Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. Journal of King Saud University – Science, 27, 224-232.

Jani NA, Azizi NAA, Aminudin N.I. (2020). Phytochemical screening and antioxidant activity of Psidium guajava. Malaysian Journal of Analytical Sciences, 24(2), 173-178

Jani NA, Sirat HM, Ahmad F, Ali N, Zainal MH. (2016). Chemical composition, antibacterial and α-glucosidase inhibitory activities of the essential oils of Neolitsea coccinea (Lauraceae). Natural Product Communications, 11(12), 1899-1902.

Jantan I, Rafi IAA, Jalil J. (2005). Platelet-activating factor (PAF) receptor-binding antagonist activity of Malaysian medicinal plants. Phytomedicine, 12, 88-92.

Jena S, Ray A, Sahoo A Panda PC, Nayak S. (2020). Deeper insight into the volatile profile of essential oil of two Curcuma species and their antioxidant and antimicrobial activities. Industrial Crops and Products, 155, 112830.

Kabera JN, Semana E, Mussa AR, He X. (2014). Plant secondary metabolites: Biosynthesis, classification, function and pharmacological properties. Journal of Pharmacy and Pharmacology, 2, 377-392.

Khanam Z, Wen CS, Bhat IUH. (2015). Phytochemical screening and antimicrobial activity of root and stem extracts of wild Eurycoma longifolia (Tongkat Ali). Journal of King Saud University-Science, 27, 23-30.

Koparde AA, Magdum C. (2017). Phytochemical studies and pharmacognostical evaluation of Zingiber cassumunar Roxb. Asian Journal of Pharmaceutical and Clinical Research, 10(10), 129-135.

Manonmani PM, Mehalingam P. (2018). Phytochemical screening and antimicrobial activity of rhizome extract of Zingiber zerumbet (L) Smith. International Journal of Botany Studies, 3(2), 57-60.

María R, Shirley M, Xavier C, Jaime S, David V, Rosa S, Jodie D. (2018). Preliminary phytochemical screening, total phenolic content and antibacterial activity of thirteen native species from Guayas province Ecuador. Journal of King Saud University – Science, 30, 500-505.

Murakami A, Takahashi D, Kinoshita T, Koshimizu K, Kim HW, Yoshihiro A, Nakamura Y, Jiwajinda S, Terao J, Ohigashi H. (2002). Zerumbone, a southeast Asian ginger sesquiterpene, markedly suppresses free radical generation: the α,β- unsaturated carbonyl group is a prerequisite. Carcinogenesis, 23(5), 795-802.

Nag A, Bandyopadhyay M, Mukherjee A. (2013). Antioxidant activities and cytotoxicity of Zingiber zerumbet (L.) Smith rhizome. Journal of Pharmacognosy and Phytochemistry, 2(3), 102-108.

Nair KPP. (2013). The agronomy and economy of turmeric and ginger. The invaluable medicinal spice crops. Elsevier.

Nik Norulaini NA, Anuar O, Omar AKM, Alkarkhi AFM, Setianto WB, Fatehah MO, Sahena F, Zaidul ISM. (2009). Optimization of SC–CO2 extraction of zerumbone from Zingiber zerumbet (L) Smith. Food Chemistry, 114, 702-705.

Prakash RO, Rabinarayan A, Kumar MS. (2011). Zingiber zerumbet L. Sm. a reservoir plant for therapeutic uses: a review. International Journal of Pharma World Research, 2(2), 1-23.

Prasad SB, Bist M, Yashwant. (2018). In vitro anti acne activity of methanolic extract of dried fruit of Embelia ribes. International Journal of Pharmaceutical Quality Assurance, 9(1), 90-94.

Ramos IL, Bandiola TMB. (2017). Phytochemical screening of Syzygium cumini (Myrtaceae) leaf extracts using different solvents of extraction. Scholars Research Library, 9(2), 74-78.

Rana VS, Ahluwalia V, Shakil NA, Prasad L. (2016). Essential oil composition, antifungal, and seedling growth inhibitory effects of zerumbone from Zingiber zerumbet. Journal of Essential Oil Research, 29, 320-329.

Rana VS, Verdeguer M, Blázquez MA. (2012). Chemical composition of the essential oil of Zingiber zerumbet var. darcyi. Natural Product Communication, 7, 1369-1370.

Riyanto S. (2007). Identification of the isolated compounds from Zingiber amaricans BL. rhizome. Indonesian Journal of Chemistry, 7(1), 93-96.

Sakinah SS, Handayani ST, Hawariah LP. (2007). Zerumbone induced apoptosis in liver cancer cells via modulation of Bax/Bcl-2 ratio. Cancer Cell International, 7, 4.

Singh CB, Chanu SB, Kh L, Swapana N, Cantrell CL, Ross SA. (2014). Chemical composition and biological activity of the essential oil of rhizome of Zingiber zerumbet (L.) Smith. Journal of Pharmacognosy and Phytochemistry, 3, 130-133.

Srivastava AK, Srivastava SK, Shah NC. (2000). Essential oil composition of Zingiber zerumbet (L.) Sm. from India. Journal of Essential Oil Research, 12, 595-597.

Sufian AS, Ramasamy K, Ahmat N, Zakaria ZA, Yusof MI. (2013). Isolation and identification of antibacterial and cytotoxic compounds from the leaves of Muntingia calabura L. Journal of Ethnopharmacology, 146(1), 198-204.

Sulaiman MR, Tengku MTA, Shaik MWM, Moin S, Yusof M, Mokhtar AF, Zakaria ZA, Israf DA, Lajis N. (2010). Antinociceptive activity of the essential oil of Zingiber zerumbet. Planta Medica, 76(2), 107-112.

Suthisut D, Fields PG, Chandrapatya A. (2011). Fumigant toxicity of essential oils from three Thai plants (Zingiberaceae) and their major compounds against Sitophilus zeamais, Tribolium castaneum and two parasitoids. Journal of Stored Products Research, 47(3), 222-230.

Tan JW, Israf DA, Tham CL. (2018). Major bioactive compounds in essential oils extracted from the rhizomes of Zingiber zerumbet (L) Smith: A mini-review on the anti-allergic and immunomodulatory properties. Frontiers in Pharmacology, 9, 652.

Tian M, Wu X, Hong Y, Wang H, Deng G, Zhou Y. (2020). Comparison of chemical composition and bioactivities of essential oils from fresh and dry rhizomes of Zingiber zerumbet (L.) Smith. BioMed Research International, 9641284.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. (2011). Phytochemical screening and extraction: a review. Internationale Pharmaceutica Sciencia, 1(1), 98-106.

Yob NJ, Jofrry SM, Affandi MM, Teh LK, Salleh MZ, Zakaria ZA. (2011). Zingiber zerumbet (L.) Smith: A review of its ethnomedicinal, chemical, and pharmacological uses. Evidence-Based Complementary and Alternative Medicine, 543216.

Yusmaniar, Wardiyah, Suprapti T, Junaedi. (2015). Antibacterial activity of the essensial oils of lempuyang wangi (Zingiber aromaticum val.), lempuyang gajah (Zingiber zerumbet sm.), and lempuyang emprit (Zingiber amaricans Bl.) on three gram negative bacteria. Asian Journal of Applied Sciences, 3(2), 290-293.

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Published

2023-02-28

How to Cite

Preshahdin, N. A., Jani, N. A., & Iberahim, R. (2023). Phytochemicals and Antibacterial Activity of Zingiber zerumbet Growing in Negeri Sembilan, Malaysia. Journal of Science and Mathematics Letters, 11(1), 20–29. https://doi.org/10.37134/jsml.vol11.1.3.2023