Biogenic Co3CuCr2O8 Nanocomposites Prepared Using Olea europaea Leaf Extract as a Nanoantibacterial Platform for Dental Caries Management
DOI:
https://doi.org/10.37134/jsml.vol14.3.6.2026Keywords:
Olea europaea , Nanotechnology, Cobalt-Copper-Chromium , nanocomposite, antibacterial , dental infectionsAbstract
The present work investigates the rapid synthesis of a Cobalt-Copper-Chromium nanocomposite by a biological method using Olea europaea leaf extract and Cobalt-Copper-Chromium salt as precursors. Cobalt-Copper-Chromium nanocomposite was successfully synthesized from O. europaea leaves extracted using a Green synthesis technique. UV-Visible Spectroscopy, Field Emission Scanning Electron Microscopy (FESEM), X-ray Diffraction (XRD), Fourier Transform Infrared (FTIR), and Transmission Electron Microscopy (TEM) were used to characterize the Cobalt-Copper-Chromium nanocomposites in detail. These measurements show that these Cobalt-Copper-Chromium nanocomposites had a spherical shape with a diameter of 45.7 to 51.6 nm and were stable for at least two months. Finally, the antibacterial activity of the Co₃CuCr₂O₈ nanocomposite was evaluated against oral pathogenic bacteria associated with dental caries, including Streptococcus mutans, Streptococcus mitis, and Staphylococcus lentus. The results demonstrated a strong concentration‑dependent inhibitory effect, with oral streptococci exhibiting higher sensitivity than S. lentus. To assess biosafety, cytotoxicity was evaluated on normal human dermal fibroblast (HDF) cells using the MTT assay after 48 h of exposure. The nanocomposite showed concentration‑dependent cytotoxicity, with high cell viability (>80%) observed at higher dilutions (≥1:80), indicating good cytocompatibility under controlled conditions. Overall, the results demonstrate that the green‑synthesized Co₃CuCr₂O₈ nanocomposite combines effective antibacterial activity with acceptable biocompatibility, supporting its potential application in dental and biomedical fields.
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References
Ahmadian E, Shahi S, Yazdani J, Dizaj SM, Sharifi S. (2018). Local treatment of the dental caries using nanomaterials. Biomedicine & Pharmacotherapy, 108, 443-447. doi:10.1016/j.biopha.2018.09.026
Al-Deen AHA, Mahmood RI, Ali SM, Mohammed-Salih HS, Jamaludin AA, Al-Obaidi JR. (2026). Green-synthesized tricalcium silicate nanoparticles from Salvia officinalis for antimicrobial coating of clear aligners and prevention of enamel demineralization. Next Nanotechnology, 9, 100427. doi:10.1016/j.nxnano.2026.100427
Engen SA, Rørvik GH, Schreurs O, Blix IJ, Schenck K. (2017). The oral commensal Streptococcus mitis activates the aryl hydrocarbon receptor in human oral epithelial cells. International Journal of Oral Science, 9, 145-150. doi:10.1038/ijos.2017.17
Esfanddarani HM, Panigrahi M. (2024). Phytosynthesis of transition (Ni, Fe, Co, Cr, and Mn) metals and their oxide nanoparticles for biomedical applications: a review. Journal of Materials Science, 59, 10677-723. doi:10.1007/s10853-024-09800-4
Fatimah S, Ragadhita R, Al Husaeni DF, Nandiyanto ABD. (2022). How to calculate crystallite size from x-ray diffraction (XRD) using Scherrer method. ASEAN Journal of Science and Engineering, 2, 65-76. doi:10.17509/ajse.v2i1.37647
Hamad AM, Atiyea QM. (2021). In vitro study of the effect of zinc oxide nanoparticles on Streptococcus mutans isolated from human dental caries. Proceedings of the Journal of Physics: Conference Series, 1879, 022041. doi:10.1088/1742-6596/1879/2/022041
Hasanin MS, Saeb MR, Abdel-Maksou MA, El-Tayeb MA, Kiani BH, Hashem AH. (2024). Advanced functional Cu-Cr-Co-NPs@ gelatin (GLN)-hydroxyethyl cellulose (HEC) nanocomposites with antimicrobial and anticancer activities. RSC Advances, 14, 37633-43. doi:10.1039/d4ra06216g
Issa HK, Maleki A, Taherizadeh A, Zargaran A. (2022). On the structure-properties relationship of amorphous and crystalline silica nanoparticles reinforced magnesium matrix nanocomposites. Journal of Alloys and Compounds, 924, 166605. doi:10.1016/j.jallcom.2022.166605
Jaafar RS, Hammood AY. (2024). Antibacterial activity and cytotoxicity of spinel copper ferrite nanoparticles synthesized by using sol gel technique and lemon juice as substrate. Pollution, 10, 63-72. doi:10.22059/poll.2023.361453.1967
Jaffar S, Taj MK, Taj I, Khan MA, Azam S, Khan S, Essote S, Samreen Z, Jaffar H. (2023). Factors responsible for dental caries among patients and its management. Pak-Euro Journal of Medical and Life Science, 4(1), 196-209. doi:10.31580/pjmls.v4iS
Jeon TH, Wu Z-Y, Chen F-Y, Choi W, Alvarez PJ, Wang H. (2022). Cobalt–copper nanoparticles on three-dimensional substrate for efficient ammonia synthesis via electrocatalytic nitrate reduction. The Journal of Physical Chemistry C, 126, 6982-6989. doi:10.1021/acs.jpcc.1c10781
Joy A, Unnikrishnan G, Megha M, Haris M, Thomas J, Kolanthai E, Muthuswamy S. (2022). Polycaprolactone/graphene oxide-silver nanocomposite: a multifunctional agent for biomedical applications. Journal of Inorganic and Organometallic Polymers and Materials, 32, 912-930. doi:10.1007/s10904-021-02180-1
Khalaf RL, Ahmed EM. (2019). Biosynthesis of silver nanoparticles using plants flowers of Nerium oleander extract and its antibacterial activity. Biochemical & Cellular Archives, 19, 263.
Khalaf RL, Ahmed EM, Mathkor TH, Al-Zubaidi HY. (2021a). Synthesis of silver nanoparticles using L. rosa flowers extracts: Thermodynamic and kinetic studies on the inhibitoty effects of nanoparticles on creatine kinase activity. Iraqi Journal of Science, 62(8), 2486-2500. doi:10.24996/ijs.2021.62.8.1
Khalaf RL, Almousawi IM, Rheima AM. (2021b). Dye pollution removal in aqueous solution using novel photochemically synthesized CoZnFe2O5 nanocomposite. Desalination and Water Treatment, 243, 287-93. doi:10.5004/dwt.2021.27892
Khudiar SS, Nayef UM, Mutlak Fa-H, Abdulridha SK. (2022). Characterization of NO2 gas sensing for ZnO nanostructure grown hydrothermally on porous silicon. Optik, 249, 168300. doi:10.1016/j.ijleo.2021.168300
Lavaee F, Motamedifar M, Rafiee G. (2022). The effect of photodynamic therapy by gold nanoparticles on Streptococcus mutans and biofilm formation: An in vitro study. Lasers in Medical Science, 37, 1717-25. doi:10.1007/s10103-021-03422-x
Lemos JA, Palmer SR, Zeng L, Wen ZT, Kajfasz JK, Freires IA, Abranches J, Brady LJ. (2019). The Biology of Streptococcus mutans. Microbiology Spectrum, 7, 1-18. doi:10.1128/microbiolspec.gpp3-0051-2018
Liu A, Liu S, Liu Y, Liu B, Liu T. (2022). Characterizing mechanical heterogeneity of coal at nano-to-micro scale using combined nanoindentation and FESEM-EDS. International Journal of Coal Geology, 261, 104081. doi:10.1016/j.coal.2022.104081
Mamidi N, Delgadillo RM, Sustaita AO, Lozano K, Yallapu MM. (2025). Current nanocomposite advances for biomedical and environmental application diversity. Medicinal Research Reviews, 45, 576-628. doi:10.1002/med.22082
Mendes CR, Dilarri G, Forsan CF, Sapata VMR, Lopes PRM, de Moraes PB, Montagnolli RN, Ferreira H, Bidoia ED. (2022). Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens. Scientific Reports, 12, 2658. doi:10.1038/s41598-022-06657-y
Mohammed-Salih HS, Ghazi A, Mahmood RI, Al-Qazzaz HH, Supian FL, Al-Obaidi JR, Jabir M. (2024). Enhancing orthodontic treatment control with fish scale-derived hydroxyapatite nanoparticles: Insights from an animal model study. The Saudi Dental Journal 36, 1128-34. doi:10.1016/j.sdentj.2024.06.007
Mohammed-Salih HS, Mahmood RI, Ghazi A, AlQrimli AF, Rashid TM, Al-Obaidi JR, Supian FL, Jabir MS. (2025). Antibacterial effect of novel orthodontic PEEK beads coated with a conjugate of fish scale hydroxy-apatite nanoparticles and GL13K peptide. BioNanoScience, 15, 557. doi:10.1007/s12668-025-02188-8
Moro D, Ulian G, Valdre G. (2021). SEM-EDS nanoanalysis of mineral composite materials: A Monte Carlo approach. Composite Structures, 259, 113227. doi:10.1016/j.compstruct.2020.113227
Pasieczna-Patkowska S, Cichy M, Flieger J. (2025). Application of Fourier transform infrared (FTIR) spectroscopy in characterization of green synthesized nanoparticles. Molecules, 30(3), 684. doi:10.3390/molecules30030684
Sami RH, Jawad SF, Zankanah FH, Jawad KH, Hasoon BA, Issa AA, Jabir MS, Al-azawi KF. (2025). Biosynthesis of platinum nanoparticles: Evaluation of their activity against streptococcus mutans and in silico study. Plasmonics, 20, 3413-3427. doi:10.1007/s11468-024-02543-7
Saratale RG, Saratale GD, Shin HS, Jacob JM, Pugazhendhi A, Bhaisare M, Kumar G. (2018). New insights on the green synthesis of metallic nanoparticles using plant and waste biomaterials: current knowledge, their agricultural and environmental applications. Environmental Science and Pollution Research, 25, 10164-10183. doi:10.1007/s11356-017-9912-6
Selvanayaki R, Rameshbabu M, Muthupandi S, Razia M, Sasi Florence S, Ravichandran K, Prabha K. (2022.) Structural, optical and electrical conductivity studies of pure and Fe doped Zinc oxide (ZnO) nanoparticles. Materials Today: Proceedings, 49, 2628-2631. doi:10.1016/j.matpr.2021.08.045
Shafey AE. (2020). Green synthesis of metal and metal oxide nanoparticles from plant leaf extracts and their applications: a review. Green Processing and Synthesis, 9(1), 304-339. doi:10.1515/gps-2020-0031
Shao Q, Feng D, Yu Z, Chen D, Ji Y, Ye Q, Cheng D. (2023). The role of microbial interactions in dental caries: Dental plaque microbiota analysis. Microbial Pathogenesis, 185, 106390. doi:10.1016/j.micpath.2023.106390
Sudha D, Kumar ER, Shanjitha S, Munshi AM, Al-Hazmi GAA, El-Metwaly NEM, Kirubavathy SJ. (2023). Structural, optical, morphological and electrochemical properties of ZnO and graphene oxide blended ZnO nanocomposites. Ceramics International, 49, 7284-7288. doi:10.1016/j.ceramint.2022.10.192
Tariq T, Ali A, Alvi F. (2026). Fabrication and characterization of Co₃O₄–Fe₂O₃–chitosan based ternary nanobiocomposite electrode for norepinephrine detection. Sensor Review, 1-10. doi:10.1108/SR-06-2025-0450
Umai D, Vikranth A, Meenambiga SS. (2021). A study on the green synthesis of silver nanoparticles from Olea europaea and its activity against oral pathogens. Materials Today: Proceedings, 44, 3647-3651. doi:10.1016/j.matpr.2020.10.681
Yadav K, Prakash S. (2017). Dental caries: A microbiological approach. Journal of Clinical Infectious Diseases & Practice, 2(1), 1-15. doi:10.4172/2476-213X.1000118
Yazdanian M, Rostamzadeh P, Rahbar M, Alam M, Abbasi K, Tahmasebi E, Tebyaniyan H, Ranjbar R, Seifalian A, Yazdanian A. (2022). The potential application of green‐synthesized metal nanoparticles in dentistry: A comprehensive review. Bioinorganic Chemistry and Applications, 2311910. doi:10.1155/2022/2311910
Zhang JS, Chu CH, Yu OY. (2022). Oral microbiome and dental caries development. Dentistry Journal, 10, 184. doi:10.3390/dj10100184
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Copyright (c) 2026 Riyam Lateef Khalaf, Mina Mohammed Kadhim, Abdalwahab Malath Alhadithi, Ola Abdul Kareem Nori
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