Acetylcholinesterase Activity of Crude Extracts and Flavonoids from Artocarpus anisophyllus Miq. and Artocarpus lowii King

Authors

  • Siti Awanis Abdullah Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, MALAYSIA
  • Siti Mariam Abdul Lathiff Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, MALAYSIA
  • Shajarahtunnur Jamil Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, MALAYSIA

DOI:

https://doi.org/10.37134/jsml.vol10.2.7.2022

Keywords:

Acetylcholinesterase, Plant Extracts, Flavonoids, Artocarpus, TLC

Abstract

A flavonoids-rich extract and seven flavonoids from the leaves and heartwoods of A. anisophyllus Miq. and  A. lowii King were investigated for their inhibitory effects against acetylcholinesterase enzyme. The flavonoids were fully characterized spectroscopically as artocarpin (1), isobavachalcone (2), pyranocycloartobiloxanthone A (3), 5,7-dihydroxy-4'-methoxy-6-prenylflavanone (4), 5-hydroxy-7,8-(2,2-dimethylchromano)-4'-methoxy-flavanone (5) 4',5-dihydroxy-6,7-(2,2-dimethylpyrano)-2'-methoxy-8-γ,γ-dimethylallylflavone (6) and 2',4'-dihydroxy-3,4-(2",2"-dimethylchromeno)-3'-prenyldihydrochalcone (7). Acetylcholinesterase inhibitory activity of the samples was determined using TLC plate with bioactivity staining based on the Ellman’s method. Pyranocycloartobiloxanthone A (3) exhibited an excellent inhibitory activity with detection limit at 7.81 µg/mL.

Downloads

Download data is not yet available.

References

Abdullah, S.A., Jamil, S., Basar, N., Lathiff, S.M.A., & Arriffin, N.M. (2016). Flavonoids from the leaves and heartwoods of Artocarpus lowii King and their bioactivities. Natural Product Research, 31(10), 1113-1120.

Azman, N.A.N., Alhawarri, M., Rawa, M.S.A., Dianita, R., Gazzali, A. M., Nogawa, T., & Wahab, H.A. (2020). Potential anti-acetylcholinesterase activity of Cassia timorensis DC. Molecules, 25(19), 4545-4559.

Alzheimer’s Association. (2013). Alzheimer’s disease Facts and Figures. Alzheimer’s & Dementia, 9, 208-245.

Ellman, G.L., Courtney, K.D., Andress, V.Jr., & Featherstone, R.M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7, 88-95.

Giovanni, S.D., Borloz, A., Urbain, A., Marston, A., Hostettmann, K., Carrupt, P.A., & Reist, M. (2008). In vitro screening assays to identify natural or synthetic acetylcholinesterase inhibitors: thin layer chromatography versus microplate methods. European Journal of Pharmaceutical Sciences, 33, 109-119.

Jamil, S., Abdullah, S.A., Lathiff, S.M.A., & Sirat, H.M. (2014). Tyrosinase inhibitory activity of flavonoids from Artocarpus lowii King. Jurnal Teknologi, 71(1), 55-58.

Jamil, S., Lathiff, S.M.A., Abdullah, S.A., Jemaon, N., & Sirat, H.M. (2014). Antimicrobial flavonoids from Artocarpus anisophyllus Miq. and Artocarpus lowii King. Jurnal Teknologi, 71(1), 95-99.

Lathiff, S.M.A., Arriffin, N.M., & Jamil, S. (2021). Phytochemicals, pharmacological and ethnomedicinal studies of Artocarpus: A scoping review. Asian Pacific Journal of Tropical Biomedicine, 11(11), 469-480.

Lathiff, S.M.A., Jemaon, N., Abdullah, S.A., & Jamil, S. (2015). Flavonoids from Artocarpus anisophyllus and their bioactivities. Natural Products Communications, 10(3), 393-396.

Nomura, T., Hano, Y., & Aida, M. (1998). Isoprenoid-substitutes flavanoids from Artocarpus plants (Moraceae). Heterocycles, 47, 1179-205.

Rhee, I.K., Meent, M.V.D., Ingkaninan, K., & Verpoorte, R. (2001). Screening for acetylcholinesterase inhibitors from amaryllidaceae using thin layer chromatography in combination with bioactivity stain. Journal of Chromatography A, 915, 217-223.

Rhee, I.K., Rijn, R.M.V., & Verpoorte, R. (2003). Qualitative determination of false-positive effects in the acetylcholinesterase assay using thin layer chromatography. Phytochemical Analysis, 14, 127-131.

Rosdi, M.N.M., Sirat, H.M., Abdullah, S.A., Jamil, S., Muhamad, I.I., & Zulkifli, R.M. (2015). Inhibitory effect of Artocarpus lowii King compounds on COX-2 and 15-LO activities. Jurnal Teknologi, 76(1), 299-302.

Vanessa, V.V., & Mah, S.H. (2021). Xanthone: Potential acetylcholinesterase inhibitor for Alzheimer’s disease treatment. Medicinal Chemistry, 21(17), 2507-2529.

Whitmore, P.J., Price, D.L., Struble, R.G., Clark, A.W., Coyle, J.T., & Delon, M.R. (1982). Alzheimer’s disease and senile dementia: Loss of neurons in the basal forebrain. Science, 215, 1237-1239.

Downloads

Published

2022-10-21

How to Cite

Abdullah, S. A., Abdul Lathiff, S. M., & Jamil, S. (2022). Acetylcholinesterase Activity of Crude Extracts and Flavonoids from Artocarpus anisophyllus Miq. and Artocarpus lowii King. Journal of Science and Mathematics Letters, 10(2), 62–66. https://doi.org/10.37134/jsml.vol10.2.7.2022

Issue

Section

Articles